TAN Shutang, Ph.D., Principal Investigator.
2020 – present Principle Investigator, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
2015 – 2020 Postdoctoral researcher, Institute of Science and Technology, Austria
2009 – 2015 DPhil of Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Science, Chinese Academy of Sciences
2005 – 2009 BSc of Science in Biotechnology, School of Life Science, Shandong University
Plants have evolved high plasticity in their growth and development owing to their sessile lifestyles. This is ensured by complex molecular networks, requiring the interplay between different signalling pathways .
Funded first by EMBO Long-Term Fellowship and IST Fellowship (Marie Currie COFUND), I started my postdoctoral research on the non-transcriptional effect of the plant hormone salicylic acid (SA) in Prof. Jiri Friml lab at IST Austria. With the advantage of state-of-the-art microscopy and live-imaging approaches, I identified a novel target of SA and uncovered the molecular mechanism underlying the developmental role of SA (Tan et al., Current Biology, 2020). Inspired by an experience of toothache, I exhibited strong interest in the function and mechanism of non-steroid anti-inflammatory drugs (NSAIDs), such as aspirin and ibuprofen, which have been developed based on SA. Indeed, SA is an ancient drug, and it was even used for curing fever and pains thousands of years ago all over the world. Though the mechanism for NSAIDs releasing inflammation seems unsolved, i.e. targeting COX-2 to inhibit prostaglandin biosynthesis, there had been no study revisiting the activity of NSAIDs in plants. Thus, I tested plants’ sensitivity to aspirin and ibuprofen, and discovered a striking twisted phenomenon caused by these two drugs. I identified one promising target of NSAIDs, TWISTED DWARF1, which might explain for part of their functions (Tan et al., Cell Reports, 2020).
Another major topic of the Tan group is the molecular regulation of PIN-FORMED (PIN)-mediated auxin transport. PINs are a family of auxin efflux carriers, and they are under multilevel regulations, including post-translational modification. In the field of auxin biology, it was thought for more than one decade that PDK1 phosphorylates PINOID, one AGC kinase responsible for PIN phosphorylation and polarity in plants. However, the exact role of PDK1 is unclear, without well-characterised fully knock-out genetic materials. By multiple approaches, I found that PDK1 has pleiotropic functions independently of PINOID. Instead, PDK1 phosphorylates a small sub-clade of AGC kinases, D6PKs, to phosphorylate PIN transporters. Notably, both PDK1 and D6PKs are basal localized, in a lipid-dependent manner, presenting a phosphoswitch for regulating PIN-mediated auxin transport and plant development (Tan et al., Nature Plants, 2020). This study adds a crucial piece to the puzzle of PIN-centred auxin transport framework.
Currently, our research work mainly focuses on:
1. Plant Cell and Developmental Biology: The ESCRT-mediated vacuolar degradation pathway.
2. Reversible protein phosphorylation: protein kinases and phosphatases involved plant developments, as well as their functions and regulations.
3. Auxin Biology: Novel regulatory factors involved in auxin biosynthesis, signaling and transport, especially PIN-mediated polar auxin transport.
4. Salicylic acid & Plant development: the developmental role of salicylic acid (SA) in plants, especially via crosstalk with the auxin network.
17. Shutang Tan, Xixi Zhang, Wei Kong, Xiao-Li Yang, Gergely Molnár, Zuzana Vondráková, Roberta Filepová, Jan Petrášek, Jiří Friml*, and Hong-Wei Xue*. The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis. Nature Plants. 2020, 6(5): 556-569.
DOI: 10.1038/s41477-020-0648-9. PMID: 32393881
16. Shutang Tan, Melinda Abas, Inge Verstraeten, Matouš Glanc, Gergely Molnár, Jakub Hajný, Pavel Lasák, Ivan Petřík, Eugenia Russinova, Jan Petrášek, Ondřej Novák, Jiří Pospíšil, and Jiří Friml*. Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants. Current Biology. 2020, 30(3): 381-395.e8.
DOI: https://doi.org/10.1016/j.cub.2019.11.058. PMID 31956021.
15. Shutang Tan, Martin Di Donato, Matouš Glanc, Xixi Zhang, Petr Klima, Noel Ferro, Aurélien Bailly, Jan Petrášek, Markus Geisler, and Jiří Friml*. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated development in plants. Cell Reports. 2020, 30(9): 108463.
14. Shutang Tan, Christian Luschnig, and Jiří Friml*. Pho-view of Auxin: Reversible Protein Phosphorylation in Auxin Biosynthesis, Transport and Signalling. Molecular Plant. 2020. S1674-2052(20)30383-X. Advance online.
DOI: https://doi.org/10.1016/j.molp.2020.11.004. PMID: 33186755
13. Krisztina Ötvös, Marco Marconi, Andrea Vega, Jose O’ Brien, Alexander Johnson, Rashed Abualia, Livio Antonielli, Juan Carlos Montesinos López, Yuzhou Zhang, Shutang Tan, Christina Artner, Jirí Friml, Rodrigo A. Gutiérrez, Krzysztof Wabnik,* and Eva Benková*. Modulation of root growth by nutrient-defined regulation of polar auxin transport. The EMBO Journal. 2020. In Press.
12. Jakub Hajný, Tomáš Prát, Nikola Rydza, Lesia Rodriguez, Shutang Tan, Inge Verstraeten, David Domjan, Ewa Mazur, Elwira Smakowska-Luzan, Wouter Smet, Eliana Mor, Jonah Nolf, BaoJun Yang, Wim Grunewald, Gergely Molnár, Youssef Belkhadir, Bert De Rybel and Jiří Friml*. Receptor kinase module targets PIN-dependent auxin transport during canalization. Science. 2020. 370(6516):550-557.
DOI: 10.1126/science.aba3178. PMID: 33122378
11. Meiyu Ke#, Zhiming Ma#, Deyan Wang, Yanbiao Sun, Chenjin Wen, Dingquan Huang, Zichen Chen, Liang Yang, Shutang Tan, Ruixi Li, Jiří Friml, Yansong Miao,* and Xu Chen*. Salicylic acid regulates PIN2 auxin transporter hyper-clustering and root gravitropic growth via Remorin-dependent lipid nanodomain organization in Arabidopsis thaliana. New Phytologist. 2020. Advance online. (#, contributed equally to the article).
DOI: 10.1111/nph.16915. PMID: 32901934
10. Hongjiang Li, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof Wabnik, Riet De Rycke, Walter A. Kaufmann, Daniel Gütl, Ricardo Tejos, Peter Grones, Meiyu Ke, Xu Chen, Jan Dettmer, and Jiří Friml*. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 2020. Advance online.
DOI: 10.1111/nph.16887. PMID: 32810889
9. Xixi Zhang, Maciek Adamowski, Petra Marhava, Shutang Tan, Yuzhou Zhang, Lesia Rodriguez, Marta Zwiewka, Vendula Pukyšová, Adrià Sans Sánchez, Vivek Raxwal, Christian Hardtke, Tomasz Nodzyński, and Jiří Friml*. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. The Plant Cell. 2020, 32(5): 1644-1664.
DOI: 10.1105/tpc.19.0086. PMID: 32193204
8. Madhumitha Narasimhan#, Alexander Johnson#, Roshan Prizak, Walter Anton Kaufmann, Shutang Tan, Barbara Casillas-Pérez, and Jiří Friml*. Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants. eLife. 2020, 9: e52067. (#, contributed equally to the article).
DOI: 10.7554/eLife.52067. PMID 31971511.
7. Yang Li#, Yaping Wang#, Shutang Tan, Matouš Glanc, Zhi Yuan, David Domjan, Kai Wang, Zhen Li, Yan Guo, Zhizhong Gong, Jiří Friml and Jing Zhang*. Root Growth Adaptation is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex. Advanced Science. 2020, 7(3): 1901455. (#, contributed equally to the article).
DOI:10.1002/advs.201901455. PMID 32042554.
6. Dingquan Huang#, Yanbiao Sun#, Zhiming Ma#, Meiyu Ke, Yong Cui, Zichen Chen, Chaofan Chen, Changyang Ji, Tuan Minh Tran, Liang Yang, Sin Man Lam, Yanhong Han, Guanghou Shui, Zhuang Wei, Shutang Tan, Kan Liao, Jiří Friml, Yansong Miao, Liwen Jiang, and Xu Chen*. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. PNAS. 2019, 116 (42): 21274-21284.
DOI: 10.1073/pnas.1911892116. PMID: 31575745
5. Marta Zwiewka#, Agnieszka Bielach#, Prashanth Tamizhselvan, Sharmila Madhavan, Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, Petre Dobrev, Rodamira Vaňková, Jiří Friml, and Vanesa B. Tognetti*. Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking. Plant and Cell Physiology. 2019, 60(2): 255-273. (#, contributed equally to the article; cover).
DOI:10.1093/pcp/pcz001. PMID 30668780.
4. Yong Tang#, Chun-Yan Zhao#, Shu-Tang Tan, and Hong-Wei Xue*. Arabidopsis Type II Phosphatidylinositol 4-Kinase PI4Kγ5 Regulates Auxin Biosynthesis and Leaf Margin Development through Interacting with Membrane-Bound Transcription Factor ANAC078. PLoS Genetics. 2016, 12(8): e1006252. (#, contributed equally to the article.)
DOI: 10.1371/journal.pgen.1006252. PMID 27529511.
3. Shu-Tang Tan, and Hong-Wei Xue*. Casein kinase 1 regulates ethylene synthesis by phosphorylating and promoting the turnover of ACS5. Cell Reports. 2014, 9(5): 1692-1702.
DOI:10.1016/j.celrep.2014.10.047. PMID 25464840.
2. Shu-Tang Tan, Cheng Dai, Hong-Tao Liu, and Hong-Wei Xue*. Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. The Plant Cell. 2013, 25(7): 2618-2632.
DOI: 10.1105/tpc.113.114322. PMID: 23897926.
1. Yong Tang, Shu-Tang Tan, and Hong-Wei Xue*. Arabidopsis inositol 1,3,4-trisphosphate 5/6 kinase 2 is required for seed coat development. Acta Biochimica et Biophysica Sinica. 2013, 45(7): 549-560.
DOI: 10.1093/abbs/gmt039. PMID: 23595027.
School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
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