Synthetic Biology Lab
School of Life Science
University of Science & Technology of China
Feb. 2009- June 2009: Visiting Scholar, University of Houston, USA
April 2008- current: Associate Professor, University of Science and Technology of China.
Aug. 2006- Feb. 2008: Postdoctoral researcher, Virginia Polytechnic Institute and State University, USA
Nov.2003-Aug. 2006: Postdoctoral researcher, Kyoto University/Ishikawa Prefectural University,Japan
March 2000- April 2003: Ph.D. Applied Molecular Microbiology, Department of Applied Life Sciences, Graduate school of Agriculture, Kyoto University, Japan.
Sept.1993- Jul.1996: M.S. Microbiochemstry, Department of Biology, Beijing Normal University, Beijing , P. R. China
Sept.1989-Jul. 1993: B.S. Biological Sciences, Department of Biology, Anhui Normal University, Anhui Province, P.R.China
We develop enable-technologies for making synthetic DNA using parallel and miniaturized platform. We are interested in methods for making DNAs (and thus RNAs) as basic elements/constructs for functional DNA/RNA clusters and proteins/protein comlexes, molecular devices of designed structures and functions, and synthetic biosystems. Our research projects include BIOCHIP-based DNA synthesis and synthetic DNA applications in RNA and protein synthesis, design and develop enzymatic systems for biosynthesis and metabolic reactions.
We are also interested in thermo-tolerant microorganisms which can be cultivated under elevated temperature (40-60oC), and can be easily handled as most mesophilic microorganisms. We focus on the research to the thermo-tolerant enzyme discovery and their application.
1. Zhang Jia, Zhang Biao, Wang Dongmei, Gao Xiaolian and Hong Jiong * , (2015). "Improving xylitol production at elevated temperature with engineered Kluyveromyces marxianus through over-expressing transporters." Bioresource Technol 175: 642-645.
2.WAN, Wen; LI, Lulu; XU, Qianqian; WANG, Zhefan; YAO, Yuan; WANG, Rongliang; ZHANG, Jia; Liu, haiyan; Gao, Xiaolian*; Hong, Jiong* (2014) Error removal in microchip-synthesized DNA using immobilized MutS. Nucleic Acids Res, 42(12),e102 doi: 10.1093/nar/gku405
3. Zhang Jia, Zhang Biao, Wang Dongmei, Gao Xiaolian, Hong Jiong* (2014) Xylitol production at high temperature by engineered Kluyveromyces marxianus. Bioresource Technol 152:192-201.
4.Zhang B, Li LL, Zhang J, Gao XL, Wang DM*, Hong J* (2013) Improving ethanol and xylitol fermentation at elevated temperature through substitution of xylose reductase in Kluyveromyces marxianus. J Ind Microbiol Biotechnol 40 (3-4):305-316.
5.Wen Wan, Dongmei Wang, Xiaolian Gao and Jiong Hong*，Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast，Appl Microbiol Biotechnol. 2011 V91(3):789-798
6.Biao Zhang, Ling Zhang, Dongmei Wang, Xiaolian Gao and Jiong Hong*，Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777，J. Ind. Microbiol. Biotechnol. 2011 V38(12)2001-2010 .
7.Jiong Hong, Ye Xinhao, Wang Yiran,Zhang Y-HP. Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Analytica Chimica Acta 2008，Vol 621，193-199.
8.Jiong Hong, Yiran Wang, Xinhao Ye, Y-H Percival Zhang, Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage, Journal of Chromatography A 2008, Vol 1194，150–154.
9.Jiong Hong, Xinhao Ye, Y-H Percival Zhang, Quantitative Determination of Cellulose Accessibility to Cellulase Based on Adsorption of a Non-Hydrolytic Fusion Protein Containing CBM and GFP with Its Applications, Langumir 2007, Vol 23, 12535-12540.
10.Jiong Hong, Yonghong Wang, Hisanori Tamaki, and Hidehiko Kumagai,Construction of thermotolerant yeast expressing thermostable cellulase genes, Journal of Biotechnol 2007 Vol. 130, 114-123.