Professor of Biochemistry and Molecular Biology, School of Life Sciences
Tel. & Fax: 0551-63600406
1) Structure-based reconstruction of the protein network in response to oxidative stress
Thanks to the methodologies of structural genomics, we will focus on solving the 3-D structures, mainly by X-ray crystallography, of the key proteins and their complexes in response to the oxidative stress in Saccharomyces cerevisiae. This will enable us to remodel the yeast protein network in response to oxidative stress, and provide us some insights into developing new drugs against aging and other oxidative stress-related diseases.
2) Structural and functional investigations of silk-related genes and proteins
Based on our previous work on fibroin gene, and taking advantage of the mass information from silkworm genome project, we will systematically study the silk-related genes and their encoded proteins, to update our knowledge on the molecular mechanism of fine regulations of replication and expression of fibroin gene, and secretion and packing of silk proteins.
1.He YX#, Huang L#, Xue Y#, Fei X, Teng YB, Rubin-Pitel S, Zhao H* and Zhou CZ*. Crystal structure and computational analyses provide insights into the catalytic mechanism of 2, 4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens. Journal of Biological Chemistry. 2010, in press
2.Teng YB, Jiang YL, He YX, Lian FM, He WW, Chen Y and Zhou CZ*. Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103. BMC Structural Biology. 2009; 9(1):67.
3.Ma XX, Jiang YL, He YX, Bao R, Chen Y, Zhou CZ*. Structures of yeast glutathione-S-transferase Gtt2 reveal a new catalytic type of GST family. EMBO Reports. 2009; 10(12):1320-1326
4.Teng YB, Ma XX, He YX, Jiang YL, Du J, Xiang C, Chen Y, Zhou CZ*. Crystal structure of Arabidopsis translation initiation factor eIF-5A2. Proteins. 2009; 77(3):736-40.
5.Bao R, Zhou CZ, Jiang C, Lin SX, Chi CW, Chen Y*. The ternary structure of the double-headed arrowhead protease inhibitor API-A complexed with two trypsins reveals a novel reactive site conformation. Journal of Biological Chemistry. 2009; 284(39):26676-84.
6.Zhang X, Hu ZY, Li WF, Li QR, Deng XJ, Yang WY, Cao Y and Zhou CZ*. Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori. BMC Molecular Biology. 2009; 10:50.
7.He YX, Gui L, Liu YZ, Du Y, Zhou Y, Li P, Zhou CZ*. Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly. Proteins. 2009; 76(1):249-54.
8.Huang L#, Sun F#, Liang C, He YX, Bao R, Liu L and Zhou CZ*. Structural basis for the unique anti-tumor activity of LZ-8 from the fungus Ganoderma lucidium. Proteins. 2009; 75(2):524-7.
9.Zhang WJ#, He YX#, Yang Z, Yu J, Chen Y, Zhou CZ*. Crystal structure of glutathione-dependent phospholipid peroxidase Hyr1 from the yeast Saccharomyces cerevisiae. Proteins. 2008; 73(4):1058-62. (*contributed equally)
10.Yu J, Zhang NN, Yin PD, Cui PX and Zhou CZ* Glutathionylation-triggered conformational changes of glutaredoxin Grx1 from the yeast Saccharomyces cerevisiae.Proteins. 2008; 72: 1077-1083