2011.1 ~ present Associate professor in School of Life Science, USTC, China
2010.2 ~ 2010.12 Staff Scientist at Beatson Institute for Cancer Research, UK
2008.6 ~ 2010.2 Postdoctorate Research Fellow at Pfizer, USA
2002.9 ~ 2008.5 Ph. D in Department of Chemistry, Johns Hopkins University, USA
1995.9 ~ 2002.7 B. Sci. and M. Sci in College of Chemistry and Molecular Engineering, Peking University, China
We are focused on the Fragment Based Lead Discovery (FBLD) targeting protein-protein interactions within the complex signal transduction network of tumor. We have accomplished the first industrial standard NMR Fragment Based Screening pipeline in China, covering from hit identification, structure-guided inhibitor evolution to cellular assays. The highly automated NMR fragment screening has been applied to a number of targets related to epigenetics and small GTPases. The high potent lead-like compounds will be employed as a small-molecule probe to protein-protein interaction network, which furthers our understanding of the molecular basis of tumor proliferation and metastasis.
We are also exploiting the application of NMR novel techniques, e.g., Residual Dipolar Couplings, Paramagnetic labelling and CEST, to large complex, e.g., nucleosome and its binding partner.
1.Ruan, K.; Tolman, J. R. "Composite Alignment Media for the Measurement of Independent Sets of NMR Residual Dipolar Couplings". J. Am. Chem. Soc. 2005, 127, 15032-15033.
2.Gebel, E. B.; Ruan, K.; Tolman, J. R.; Shortle, D. "Multiple Alignment Tensors from a Denatured Protein". J. Am. Chem. Soc. 2006, 128, 9310-9311.
3.Tolman, J. R.; Ruan, K. "NMR Residual Dipolar Couplings as Probes of Biomolecular Dynamics". Chemical Reviews 2006, 106, 1720-1736.
4.Ruan, K.; Wang B.-H., Xiao J.-X., et al. "Interfacial tension of aqueous three-phase systems formed by triton X-100/PEG/dextran". J. Disp. Sci. & Tech. 2006, 27, 927-930.
5.Ruan, K.; Briggman, K. B.; Tolman,J. R. "De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media". Journal of Biom. NMR 2008, 41, 61-76.
6.Ruan, K.; Yang, S.; Van Sant K. A.; Likos, J. J. “Application of Hadamard Spectroscopy to Automated Combined Verification in High-throughput NMR”. Magnetic Resonance in Chemistry 2009, 47, 693-700.
7.Norcross,J. A.; Milling C. T.; Olson, D. L.; Xu, D.; Audrieth, A.; Albrecht, R.; Ruan, K.; Likos, J.; Jones, C.; Peck, T. L.; “Multiplexed NMR: An Automated CapNMR Dual-Sample Probe”. Analytical Chemistry 2010, 82, 7227–7236.
8.He, C; Pillai, S; Taglini, F; Li, FD; Ruan, K; Zhang, JH; Wu, JH; *Shi, YY; Bayne, EH. “Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification”, PNAS, 110(21), 1879-1888, 2013.
9.Dai, YN; Chi, CB; Zhou, K; Cheng, W; Jiang, YL; Ren, YM; Ruan, K; Chen, YX; *Zhou, CZ. “Structure and catalytic mechanism of yeast 4-amino-4-deoxychorismate lyase”, Journal of Biological Chemistry, 288, 22985-22992, 2013.
10.Gao, J; Ma, RS; Wang, W; Wang, N; Sasaki, R; Snyderman, D; Wu, JH; *Ruan, K. “Automated NMR Fragment Based Screening Identified a Novel Interface Blocker to the LARG/RhoA Complex”, Plos One, 9(2), e88098, 2014.
11.Liu, ZH; Li, FD; Ruan, K; Zhang, JH; Mei, YD; *Wu, JH; Shi, YY. “Structural and Functional Insights into the Human Borjeson-Forssman-Lehmann Syndrome- associated Protein PHF6”, Journal of Biological Chemistry, 289(14), 10069-10083, 2014.