Professor of School of Life Sciences
University of Science & Technology of China
HuangShan Road #443，Anhui Province, Hefei City, 230027
Tel: 86(551) 3607812 (o)
1991-1996 B.S. in Biochemistry and Molecular Biology, University of Science and Technology of China (USTC), China
1996-1999 M.S. in Biochemistry and Molecular Biology, University of Science and Technology of China (USTC), China
1999-2004 Ph.D. in Cancer Biology, McArdle Laboratory for Cancer Research, University of Wisconsin, Madison
2005-2010 Postdoctoral fellow in Department of Pharmacology and Department of Genetics, University of Wisconsin, Madison
2011-present Professor, School of Life Sciences, University of Science and Technology of China
The mechanistic underpinnings of RNAi are broadly conserved across eukaryotes. Initial successes utilizing small RNAs to target oncogenic and viral mRNAs have generated excitement that small RNAs may eventually be utilized to treat human diseases. Prior to the rational use of small RNA in therapeutics, it is essential to understand their biogenesis, specificity, transportation, and endogenous roles.
We am very interested in how small RNAs are transported and regulated, and how they function in the nucleus in metazoan. To address these questions, we conducted a genetic screen to identify factors required for nuclear RNAi in the model organism C. elegans and have identified three new genes termed nuclear RNAi defective (NRDE)-1/2/3. We have also identified a bifurcation of the nuclear and cytoplasmic RNAi pathway, a novel small RNA transport pathway, and a novel nuclear gene silencing mechanism.
Our research indicates that metazoans use a different mechanism than A. thaliana and S. pombe to silence gene expression in the nucleus. Further understanding how small RNAs function in the nucleus via NRDEs may permit more stable and specific inhibition of gene expression and facilitate advancement of both basic research and therapeutics.
1.Burkhart, K.B., Guang, S., Bochner, A.F., and Kennedy, S., A genetic screen identifies nrde-1 and nrde-4, two novel nuclear RNAi factors. (Accepted)
2.Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Burton, N., and Kennedy, S., (2010) Small regulatory RNAs inhibit RNA Polymerase II during the elongation phase of transcription. Nature, 465:1097-1101 Commented by: (1) Heinrichs, A., (2010) Nature Reviews Molecular Cell Biology 11:539.
3.Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Harding, S., Lachowiec, J., and Kennedy, S., (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science 321:537-541 Research Article Commented by: (1) Meister, G., (2008) Science 321:496-497. (2) Heinrichs, A., (2008) Nature Reviews Molecular Cell Biology 9:666.
4.Guang, S., Felthauser, A., and Mertz, J. (2005) Binding of hnRNP L to the pre-mRNA processing enhancer (PPE) of herpes simplex virus’ thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol. Cell. Biol. 25:6303-6313.
5.Guang, S. and Mertz, J.E. (2005) PPE-like elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes. Nucleic Acid Res. 33(7):2215-2226.