2014.04.23, Professor Sun Fei Published a paper entitled:“Phosphorylation of CDK2 on Threonine 160 Influences Silencing of Sex Chromosome During Male Meiosis” in Biology of Reproduction,online

  • Author:Wang Lu、Liu Wenjing、Zhao Weidong、Song Gendi、Wang Guishuan、Wang Xiaorong、Sun Fei
    In mammalian meiosis, the X and Y chromosomes are largely unsynapsed and transcriptionally silenced during the pachytene stage of meiotic prophase (meiotic sex chromosome inactivation), forming a specialized nuclear territory called sex or XY body. An increasing number of proteins and non-coding RNAs were found to localize to the sex body and take part in influencing expression of sex chromosome genes. Cdk2 (cyclin-dependent kinase 2)-/- spermatocytes show incomplete sex chromosome pairing. Here, we further show that p-CDK239 (phosphorylation of CDK2 isoform 1 (39 kD) on threonine 160) localizes to the sites of asynapsis and the sex body, interacting with phosphorylated H2AX (gamma-H2AX). Meanwhile, p-CDK239 is frequently mislocalized throughout the sex body and meiotic sex chromosome inactivation is disrupted in PWK×C57BL/6J hybrid mice. Furthermore, pachytene spermatocytes treated with mevastatin (an inhibitor of p-CDK2) showed overexpression of sex chromosome-linked genes. Our results highlight an important role for p-CDK239 in influencing silencing of the sex chromosomes during male meiosis by interacting with gamma-H2AX. 
    DOI: 10.1095/biolreprod.113.116624


  • more