2011.08.18,Professor Wang Guanghui Published a paper entitled “Oxidative DJ-1 interacts with mitochondrial protein BCL-XL” in JBC

  • Author:Fu Haigang,Fu Kai,Wang Dan,Mu chenchen,Wang Guanghui

    Abstract

    Parkinson disease (PD)- and cancer-associated protein, DJ-1, mediates cellular protection via many signaling pathways. Deletions or mutations in the DJ-1 gene are directly linked to autosomal recessive early-onset PD. DJ-1 has potential roles in mitochondria. Here, we show that DJ-1 increases its mitochondrial distribution in response to ultraviolet B (UVB) irradiation and binds to Bcl-XL. The interactions between DJ-1 and Bcl-XL are oxidation-dependent. DJ-1(C106A), a mutant form of DJ-1 that is unable to be oxidized, binds Bcl-XL much less than DJ-1 does. Moreover, DJ-1 stabilizes Bcl-XL protein level by inhibiting its ubiquitination and degradation through ubiquitin proteasome system (UPS) in response to UVB irradiation. Furthermore, under UVB irradiation, knockdown of DJ-1 leads to increases of Bcl-XL ubiquitination and degradation upon UVB irradiation, thereby increasing mitochondrial Bax, caspase-3 activation and PARP cleavage. These data suggest that DJ-1 protects cells against UVB-induced cell death dependent on its oxidation and its association with mitochondrial Bcl-XL.

    doi: 10.1074/jbc.M110.207134

     

     

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