2011.07.18,Professor Wu Mian in PNAS Published a paper in PNAS

  • 2011.07.18,Professor Wu Mian published a paper entitled “Siva1 suppresses epithelial-mesenchymal transition and metastasis of tumor cells by inhibiting stathmin and stabilizing microtubules” in PNAS
    Author:Li Nan、Jiang Peng、Du Wenjing、Wu Zhengkai、Li cong、Qiao Meng rang、Yang Xiaolu、Wu Mian 

    Abstract:

    Epithelial–mesenchymal transition (EMT) enables epithelial cells to acquire motility and invasiveness that are characteristic of mesenchymal cells. It plays an important role in development and tumor cell metastasis. However, the mechanisms of EMT and their dysfunction in cancer cells are still not well understood. Here we report that Siva1 interacts with stathmin, a microtubule destabilizer. Siva1 inhibits stathmin’s activity directly as well as indirectly through Ca2+/calmodulin-dependent protein kinase II (CaMK II)-mediated phosphorylation of stathmin at Ser16. Via the inhibition of stathmin, Siva1 enhances the formation of microtubules and impedes focal adhesion assembly, cell migration, and EMT. Low levels of Siva1 and Ser16-phosphorylated stathmin correlate with high metastatic states of human breast cancer cells. Knockdown of Siva1 promotes cancer dissemination in mouse models. These results suggest that microtubule dynamics are critical for EMT. Furthermore, they reveal an important role for Siva1 in suppressing cell migration and EMT and indicate that down-regulation of Siva1 may contribute to tumor cell metastasis.

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