2011.02.28,Professor Tu Xiaoming published a paper entitled ”Solution structure of Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae” in Biochemical Journal

  • Author:Zhang Wen、Zhang Jiahai、Zhang Xuecheng、Xu Chao、Tu Xiaoming
    Abstract
    Chromatin modifications play important roles in cellular biological process. Recently, a novel conserved domain family, YEATS, was discovered in a variety of eukaryotic species ranging from yeast to human. Taf14, which is involved in a few protein complexes of chromatin remodeling and gene transcription, and essential for keeping chromosome stability, regular cell growth and transcriptional regulation, contains a YEATS domain at its N-terminus. In the present study, we determined the solution structure of Taf14 YEATS domain using NMR spectroscopy. Taf14 YEATS domain adopts a global fold of an elongated β-sandwich, similar to Yaf9 YEATS domain. Meanwhile, Taf14 YEATS domain differs significantly from Yaf9 YEATS domain in some aspects, which might indicate different structural classes of YEATS domain family. Functional studies indicate that YEATS domain is critical for the function of Taf14 in inhibiting cell growth under stress conditions. In addition, our results show that the C-terminus of Taf14 is responsible for its interaction with Sth1, which is an essential component of the RSC complex. All these data imply that Taf14 is involved in transcriptional activation of Saccharomyces cerevisiae and the YEATS domain of Taf14 might play a negative role in cell growth.


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