2014.5.14,Professor Sun Fei Published a paper in The Journal of Biological Chemistry,online
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    2014.5.14,Professor Sun Fei Published a paper in The Journal of Biological Chemistry,online

  • 2014.5.14,Professor Sun Fei Published a paper entitled:“Transactivation of miR-320 by miR-383 regulates granulosa cell functions by targeting E2F1 and SF” in The Journal of Biological Chemistry,online
    Author:Yin Mianmian、Wang Xiaorong、Yao Guidong、Lv Mingrong、Liang Meng、Sun Yingpu、Sun Fei
    Abstract:
    Our previous studies have shown that miR-320 is one of the most down-regulated miRNAs in mouse ovarian granulosa cells (GCs) after TGF-β1 treatment. However, the underlying mechanisms of miR-320 involved in GC function during follicular development remain unknown. In this study, we found that PMSG treatment resulted in the suppression of miR-320 expression in a time-dependent manner. miR-320 was mainly expressed in GCs and oocytes of mouse ovarian follicles in follicular development. Overexpression of miR-320 inhibited estradiol (E2) synthesis and proliferation of GCs through targeting E2F1 and SF-1. E2F1/SF-1 mediated miR-320-induced suppression of GC proliferation and of GC steroidogenesis. FSH down-regulated the expression of miR-320 and regulated the function of miR-320 in mouse GCs. miR-383 promoted the expression of miR-320 and enhanced miR-320-mediated suppression of GC proliferation. Injection of miR-320 into the ovaries of mice partially promoted the production of testosterone and progesterone, but inhibited E2 release in vivo. Moreover, the expression of miR-320 and miR-383 was up-regulated in the follicular fluid of polycystic ovarian syndrome (PCOS) patients, while the expression of E2F1 and SF-1 was down-regulated in GCs. These data demonstrated that miR-320 regulates the proliferation and steroid production by targeting E2F1 and SF-1 in the follicular development. Understanding the regulation of miRNA biogenesis and function in the follicular development will potentiate the usefulness of miRNA in the treatment of reproduction and some steroid-related disorders.

    DOI: 10.1074/jbc.M113.546044

     

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